Perkin-Elmer Lambda 25 UV-Vis Spectrophotometer

UV-Vis spectroscopy observes transitions in electrons from the ground state to the excited state following absorption of UV and visible light. This is an important instrument in analytical chemical as it allows for quantitative determination of different analytes, including transition metal ions, conjugated organic compounds and biomolecules.

UV/Vis spectroscopy can be used to determine the concentration of an analyte in solution based on the Beer-Lambert law indicating that, within a certain range of concentrations, the concentration is directly proportional to the absorbance.

         A=εbC

• A=Absorbance
• ε=Molar Absorptivity, or extinction coefficient
• b=pathlength
• C=Concentration

 

Instrument Specifications

Wavelength range: 190-1100 nm
Bandwidth: 1 nm fixed
Wavelength accuracy (At D2 peak (656.1 nm)): ±0.1 nm
Wavelength reproducibility (10 measurements at 656.1 nm): ±0.05
Construction: Solid CNC-machined aluminum chassis for thermal and vibration stability
Optics: Double-beam, sealed, quartz-coated mirrors; lens-free system to reduce chromatic aberrations
Monochromator: Seya Namioka
Grating: Holographic, concave grating with 1053 lines per mm
Source: Deuterium and Tungsten prealigned sources with automatic switch-over