{"id":588,"date":"2021-10-14T04:55:35","date_gmt":"2021-10-14T04:55:35","guid":{"rendered":"https:\/\/commons.ggc.edu\/chemistry\/?page_id=588"},"modified":"2021-10-14T04:55:35","modified_gmt":"2021-10-14T04:55:35","slug":"varian-cary-uv-vis-50","status":"publish","type":"page","link":"https:\/\/commons.ggc.edu\/chemistry\/research\/instrumentation\/varian-cary-uv-vis-50\/","title":{"rendered":"Varian Cary UV-Vis 50"},"content":{"rendered":"<p>UV-Vis spectroscopy observes transitions in electrons from the ground state to the excited state following absorption of UV and visible light. This is an important instrument in analytical chemical as it allows for quantitative determination of different analytes, including transition metal ions, conjugated organic compounds and biomolecules.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"\" src=\"https:\/\/orbitbiotech.com\/wp-content\/uploads\/2018\/04\/uv-spectro-Orbit-Biotech-Training.jpg\" alt=\"Molecular analysis using UV\/Visible spectroscopy\" width=\"499\" height=\"208\" \/><\/p>\n<p>UV\/Vis spectroscopy can be used to determine the concentration of an analyte in solution based on the Beer-Lambert law indicating that, within a certain range of concentrations, the concentration is directly proportional to the absorbance.<\/p>\n<p><em>\u00a0 \u00a0 \u00a0 \u00a0 \u00a0A=\u03b5bC<\/em><\/p>\n<ul>\n<li><em>A<\/em>=Absorbance<\/li>\n<li>\u03b5=Molar Absorptivity, or extinction coefficient<\/li>\n<li><em>b<\/em>=pathlength<\/li>\n<li>C=Concentration<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>UV-Vis spectroscopy observes transitions in electrons from the ground state to the excited state following absorption of UV and visible light. This is an important instrument in analytical chemical as it allows for quantitative determination of different analytes, including transition metal ions, conjugated organic compounds and biomolecules. UV\/Vis spectroscopy can be used to determine the concentration of an analyte in solution based on the Beer-Lambert law indicating that, within a certain range of concentrations, the concentration is directly proportional to&#8230;<\/p>\n<p class=\"read-more\"><a class=\"btn btn-default\" href=\"https:\/\/commons.ggc.edu\/chemistry\/research\/instrumentation\/varian-cary-uv-vis-50\/\"> Read More<span class=\"screen-reader-text\">  Read More<\/span><\/a><\/p>\n","protected":false},"author":153,"featured_media":0,"parent":109,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"jetpack_post_was_ever_published":false,"footnotes":""},"class_list":["post-588","page","type-page","status-publish","hentry","user-has-not-earned"],"jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/PcrxoI-9u","jetpack-related-posts":[{"id":583,"url":"https:\/\/commons.ggc.edu\/chemistry\/research\/instrumentation\/genesys-30-uv-vis-spectrometer\/","url_meta":{"origin":588,"position":0},"title":"Genesys 30 UV-Vis Spectrometer","author":"mkirberger","date":"October 14, 2021","format":false,"excerpt":"UV-Vis spectroscopy observes transitions in electrons from the ground state to the excited state following absorption of UV and visible light. 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