{"id":534,"date":"2021-10-08T04:05:38","date_gmt":"2021-10-08T04:05:38","guid":{"rendered":"https:\/\/commons.ggc.edu\/chemistry\/?page_id=534"},"modified":"2021-10-14T04:52:36","modified_gmt":"2021-10-14T04:52:36","slug":"perkin-elmer-lambda-25-uv-vis","status":"publish","type":"page","link":"https:\/\/commons.ggc.edu\/chemistry\/research\/instrumentation\/perkin-elmer-lambda-25-uv-vis\/","title":{"rendered":"Perkin-Elmer Lambda 25 UV-Vis"},"content":{"rendered":"<p><img decoding=\"async\" class=\"alignleft\" src=\"http:\/\/www.selectscience.net\/images\/products\/7941_Lambda-25-35-45.jpg\" alt=\"LAMBDA 25, 35, &amp;amp; 45 UV\/Vis Spectrophotometers from PerkinElmer, Inc. | SelectScience\" \/><strong>Perkin-Elmer Lambda 25 UV-Vis Spectrophotometer<\/strong><\/p>\n<p>UV-Vis spectroscopy observes transitions in electrons from the ground state to the excited state following absorption of UV and visible light. This is an important instrument in analytical chemical as it allows for quantitative determination of different analytes, including transition metal ions, conjugated organic compounds and biomolecules.<\/p>\n<p>UV\/Vis spectroscopy can be used to determine the concentration of an analyte in solution based on the Beer-Lambert law indicating that, within a certain range of concentrations, the concentration is directly proportional to the absorbance.<\/p>\n<p><em>\u00a0 \u00a0 \u00a0 \u00a0 \u00a0A=\u03b5bC<\/em><\/p>\n<ul>\n<li><em>A<\/em>=Absorbance<\/li>\n<li>\u03b5=Molar Absorptivity, or extinction coefficient<\/li>\n<li><em>b<\/em>=pathlength<\/li>\n<li>C=Concentration<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>Instrument Specifications<\/strong><\/p>\n<p>Wavelength range: 190-1100 nm<br \/>\nBandwidth: 1 nm fixed<br \/>\nWavelength accuracy (At D2 peak (656.1 nm)): \u00b10.1 nm<br \/>\nWavelength reproducibility (10 measurements at 656.1 nm): \u00b10.05<br \/>\nConstruction: Solid CNC-machined aluminum chassis for thermal and vibration stability<br \/>\nOptics: Double-beam, sealed, quartz-coated mirrors; lens-free system to reduce chromatic aberrations<br \/>\nMonochromator: Seya Namioka<br \/>\nGrating: Holographic, concave grating with 1053 lines per mm<br \/>\nSource: Deuterium and Tungsten prealigned sources with automatic switch-over<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Perkin-Elmer Lambda 25 UV-Vis Spectrophotometer UV-Vis spectroscopy observes transitions in electrons from the ground state to the excited state following absorption of UV and visible light. This is an important instrument in analytical chemical as it allows for quantitative determination of different analytes, including transition metal ions, conjugated organic compounds and biomolecules. UV\/Vis spectroscopy can be used to determine the concentration of an analyte in solution based on the Beer-Lambert law indicating that, within a certain range of concentrations, the&#8230;<\/p>\n<p class=\"read-more\"><a class=\"btn btn-default\" href=\"https:\/\/commons.ggc.edu\/chemistry\/research\/instrumentation\/perkin-elmer-lambda-25-uv-vis\/\"> Read More<span class=\"screen-reader-text\">  Read More<\/span><\/a><\/p>\n","protected":false},"author":153,"featured_media":0,"parent":109,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"jetpack_post_was_ever_published":false,"footnotes":""},"class_list":["post-534","page","type-page","status-publish","hentry","user-has-not-earned"],"jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/PcrxoI-8C","jetpack-related-posts":[{"id":583,"url":"https:\/\/commons.ggc.edu\/chemistry\/research\/instrumentation\/genesys-30-uv-vis-spectrometer\/","url_meta":{"origin":534,"position":0},"title":"Genesys 30 UV-Vis Spectrometer","author":"mkirberger","date":"October 14, 2021","format":false,"excerpt":"UV-Vis spectroscopy observes transitions in electrons from the ground state to the excited state following absorption of UV and visible light. 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